Regulatory

Part:BBa_M50045

Designed by: Mark Buckup   Group: Stanford BIOE44 - S11   (2016-12-08)


hCMV Promoter and Kozak

The hCMV promoter is usually conducted in mammalian cells, as it is a strong promoter and drives constitutive expression of genes under its control. The kozak sequence here plays a major role in the translation process for eukaryotes. The elements in between help with overall transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1052
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 888


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Categories
Parameters
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